Ultimately the boys’ failure to even help her only cements the impact of what has been done in viewers' memories. The depth of shadows in the scene, the pain in the creature's voice, and the slow but increasing anger in Ed’s countenance combine to make a powerful, and horrible moment that has stuck in the minds of fans and even wormed its way into popular meme culture. Shou Tucker created one of the darkest moments in anime, and the creators of the show delivered it with devastating precision. Especially contrasted with the strained, but idealistic image that was first presented in his life. His complete and utter lack of humanity and empathy cements him as one of the most vile villains in the show. However, when finally forced to confront the truth Shou shows no remorse, he views it as a necessary step in the process and something that anyone in his position would have and should have, done. On its own, the horror of a man sacrificing first his wife, and then his young daughter in the pursuit of a title would be enough to cement this as one of the darkest moments of anime. Almost immediately, one user posted an image of the Nina-Alexander chimera, which left even other channers more than a little uncomfortable. In a thread on the /a/ board, 4chan users anonymously posted a thread about anime animal noises. What the viewers may or may not have already suspected is confirmed by Ed, Shou transmuted his daughter and her dog together to create a new creature that had some of the mental faculties and memories of the human, but obviously wasn’t the same happy child that they fell in love with. To the surprise of no one, this meme started on 4chan.
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